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Effect of S. nepalensis on the levels <t>of</t> <t>InsR/PI3K/Akt/HIF-1α</t> pathway proteins in livers of NAFLD-affected mice (n = 3).
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Effect of S. nepalensis on the levels of InsR/PI3K/Akt/HIF-1α pathway proteins in livers of NAFLD-affected mice (n = 3).

Journal: Scientific Reports

Article Title: Deciphering the hepatoprotective mechanism of Sarcopyramis nepalensis wall against non-alcoholic fatty liver disease by network pharmacology and experimental validation

doi: 10.1038/s41598-025-29480-7

Figure Lengend Snippet: Effect of S. nepalensis on the levels of InsR/PI3K/Akt/HIF-1α pathway proteins in livers of NAFLD-affected mice (n = 3).

Article Snippet: Primary antibodies against InsR (20433-1-AP, Proteintech, China), p-PI3K (YP0224, Immunoway, USA), PI3K (60225-1-Ig, Proteintech, China), p-Akt (YP0006, Immunoway, USA), Akt (YT0185, Immunoway, USA), HIF-1α (BF8002, Affinity, Melbourne), and β-actin (66009-1-Ig, Proteintech, China) were used.

Techniques:

Effect of FTY720 on the mRNA expression levels of proteins engaged in insulin signaling (IGF-1, INSR, and IDE) measured using real-time PCR in the brain cortex and hippocampus of standard (SD) mice, streptozotocin (STZ) mice, and STZ mice administered with FTY720 (STZ + FTY). * p < 0.05; ** p < 0.01 as compared to the appropriate controls ( n = 17 for each gene, 5–6 for each group); one-way ANOVA with Tukey post hoc test.

Journal: Biomolecules

Article Title: Fingolimod Improves Anxiety-like Behavior and Modulates Sphingosine-1-Phosphate Receptors Gene Expression in a Diabetic Mouse Model

doi: 10.3390/biom15111485

Figure Lengend Snippet: Effect of FTY720 on the mRNA expression levels of proteins engaged in insulin signaling (IGF-1, INSR, and IDE) measured using real-time PCR in the brain cortex and hippocampus of standard (SD) mice, streptozotocin (STZ) mice, and STZ mice administered with FTY720 (STZ + FTY). * p < 0.05; ** p < 0.01 as compared to the appropriate controls ( n = 17 for each gene, 5–6 for each group); one-way ANOVA with Tukey post hoc test.

Article Snippet: Real-time PCR was performed on Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific, Inc., Waltham, MA, USA) using TaqMan Fast Advanced Master Mix, following the manufacturer’s guidelines and using specific mouse primers (Applied Biosystems, Life Technologies Corporation life technologies corporation, Pleasanton, CA, USA): Sphk1 (Mm00448841_g1), Sphk2 (Mm00445021_m1) S1pr1 (Mm02619656_s1), S1pr3 (Mm02620181_s1), Il6 (Mm00446190_m1), Tnf (Mm00443258_m1), Ide (Mm00473077_m1), Igf1 (Mm00439560_m1), Insr (Mm01211875_m1), Slc2a3 (Mm00441483_m1), Slc2a4 (Mm00436615_m1), Sirt1 (Mm01168521_m1).

Techniques: Expressing, Real-time Polymerase Chain Reaction